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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
|
pubmed:dateCreated |
1981-2-26
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pubmed:abstractText |
A method for preparing reproducible chromatin fractions is described. Fragmentation is accomplished by expelling whole chromatin from an enclosed stainless-steel chamber through a restricted orifice utilizing low nitrogen pressure. Sheared chromatin is fractionated into two distinct heavy and light fractions by centrifugation on 5-20% linear sucrose gradient.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0032-7484
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
511-9
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading | |
pubmed:year |
1980
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pubmed:articleTitle |
A nitrogen pressure-shearing apparatus for preparing distinct populations of chromatin fractions.
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pubmed:publicationType |
Journal Article
|