| pubmed-article:7442253 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7442253 | lifeskim:mentions | umls-concept:C0026339 | lld:lifeskim |
| pubmed-article:7442253 | lifeskim:mentions | umls-concept:C0025202 | lld:lifeskim |
| pubmed-article:7442253 | lifeskim:mentions | umls-concept:C0597519 | lld:lifeskim |
| pubmed-article:7442253 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:7442253 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7442253 | pubmed:dateCreated | 1981-2-24 | lld:pubmed |
| pubmed-article:7442253 | pubmed:abstractText | The transplantable B-16 melanotic melanoma carried in syngeneic C57Bl/6J female mice and the Syrian hamster melanoma cell line, RPMI 3460, were utilized to determine whether steroid-hormone receptors are present in animal melanomas. In the B-16 melanoma, a cytoplasmic-estrogen receptor is detectable, but there is no evidence for androgen or progestin receptors. Some tumors contain a glucocorticoid-binding macromolecule. Sucrose-density gradient centrifugation of cytosol after incubation with [3H]-estradiol revealed an 8S peak that was suppressed by excess radioinert diethylstilbesterol. Binding varied from 5-35 fmoles per mg cytosol protein. Scatchard analysis of [3H]-estradiol binding in cytosol yielded a single class of high-affinity binding sites; the dissociation constant is 6 x 10(-10) M. The receptor molecule is shown to be estrogen-specific by ligand competition assays. In contrast to B-16 melanoma, no estrogen, androgen, or progestin receptor can be found in the Syrian hamster melanoma cell line. However, a substantial level of specific binding is observed using [3H]-dexamethasone. Sucrose-gradient centrifugation of cytosol from this cell line after incubation with [3H]-dexamethasone revealed a 7S peak that was suppressed by excess radioinert dexamethasone. Scatchard analysis indicated a single class of high-affinity sites with a dissociation constant of 2 x 10(-9) M. Binding levels from 70-610 fmoles per mg cytosol protein were observed. The Syrian hamster melanoma cells also exhibit a biological response to glucocorticoids: Dexamethasone causes both an inhibition of growth and a decrease in final-cell density in these cells. | lld:pubmed |
| pubmed-article:7442253 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7442253 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7442253 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7442253 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7442253 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7442253 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7442253 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7442253 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7442253 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7442253 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7442253 | pubmed:issn | 0091-7419 | lld:pubmed |
| pubmed-article:7442253 | pubmed:author | pubmed-author:HornDD | lld:pubmed |
| pubmed-article:7442253 | pubmed:author | pubmed-author:MarklandF... | lld:pubmed |
| pubmed-article:7442253 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7442253 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:7442253 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7442253 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7442253 | pubmed:pagination | 35-46 | lld:pubmed |
| pubmed-article:7442253 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7442253 | pubmed:meshHeading | pubmed-meshheading:7442253-... | lld:pubmed |
| pubmed-article:7442253 | pubmed:meshHeading | pubmed-meshheading:7442253-... | lld:pubmed |
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| pubmed-article:7442253 | pubmed:meshHeading | pubmed-meshheading:7442253-... | lld:pubmed |
| pubmed-article:7442253 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:7442253 | pubmed:articleTitle | Steroid hormone receptor studies in melanoma model systems. | lld:pubmed |
| pubmed-article:7442253 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7442253 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7442253 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:7442253 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
