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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1981-2-24
|
| pubmed:abstractText |
The transplantable B-16 melanotic melanoma carried in syngeneic C57Bl/6J female mice and the Syrian hamster melanoma cell line, RPMI 3460, were utilized to determine whether steroid-hormone receptors are present in animal melanomas. In the B-16 melanoma, a cytoplasmic-estrogen receptor is detectable, but there is no evidence for androgen or progestin receptors. Some tumors contain a glucocorticoid-binding macromolecule. Sucrose-density gradient centrifugation of cytosol after incubation with [3H]-estradiol revealed an 8S peak that was suppressed by excess radioinert diethylstilbesterol. Binding varied from 5-35 fmoles per mg cytosol protein. Scatchard analysis of [3H]-estradiol binding in cytosol yielded a single class of high-affinity binding sites; the dissociation constant is 6 x 10(-10) M. The receptor molecule is shown to be estrogen-specific by ligand competition assays. In contrast to B-16 melanoma, no estrogen, androgen, or progestin receptor can be found in the Syrian hamster melanoma cell line. However, a substantial level of specific binding is observed using [3H]-dexamethasone. Sucrose-gradient centrifugation of cytosol from this cell line after incubation with [3H]-dexamethasone revealed a 7S peak that was suppressed by excess radioinert dexamethasone. Scatchard analysis indicated a single class of high-affinity sites with a dissociation constant of 2 x 10(-9) M. Binding levels from 70-610 fmoles per mg cytosol protein were observed. The Syrian hamster melanoma cells also exhibit a biological response to glucocorticoids: Dexamethasone causes both an inhibition of growth and a decrease in final-cell density in these cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0091-7419
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
13
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
35-46
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7442253-Animals,
pubmed-meshheading:7442253-Binding, Competitive,
pubmed-meshheading:7442253-Cell Division,
pubmed-meshheading:7442253-Cell Line,
pubmed-meshheading:7442253-Cricetinae,
pubmed-meshheading:7442253-Cytosol,
pubmed-meshheading:7442253-Dexamethasone,
pubmed-meshheading:7442253-Estradiol,
pubmed-meshheading:7442253-Female,
pubmed-meshheading:7442253-Kinetics,
pubmed-meshheading:7442253-Melanoma,
pubmed-meshheading:7442253-Mesocricetus,
pubmed-meshheading:7442253-Mice,
pubmed-meshheading:7442253-Mice, Inbred C57BL,
pubmed-meshheading:7442253-Neoplasms, Experimental,
pubmed-meshheading:7442253-Receptors, Estrogen,
pubmed-meshheading:7442253-Receptors, Steroid,
pubmed-meshheading:7442253-Skin
|
| pubmed:year |
1980
|
| pubmed:articleTitle |
Steroid hormone receptor studies in melanoma model systems.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|