Linked Life Data

7440561

Source:http://linkedlifedata.com/resource/pubmed/id/7440561

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1981-2-19
pubmed:abstractText
Deuterium nuclear magnetic resonance spectra (at 55.3 MHz) have been obtained of 19F-2H double-labeled phospholipids in pure lipid bilayers, and of 2H-labeled lipid in a 19F-labeled bilayer, as a function of concentration, to assess the perturbing influence of 19F sites in lipid hydrocarbon chains. Order parameters of 2H-labeled sites adjacent to C-8 myristic fluorine probes in pure lipid bilayers, and 19F spin label order parameters themselves, are about 30% lower than those deduced from the use of nonperturbing 2H probes. The effect is intramolecular rather than intermolecular and presumably represents increased gauche states due to the increased size of the 19F label. This effect is consistent with the view that difluoromethylene fatty acyl chains function in a manner approximating that of unsaturated fatty acyl chains. The differences disappear in the presence of cholesterol at very high order parameters (Smol approximately 0.8 to 0.9). These results represent the first attempt at elucidating the perturbing effects of a high sensitivity probe (19F) and indicate that caution must be used when using spectroscopic probes to deduce the absolute magnitude of hydrocarbon chain order parameters.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
255
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11652-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Deuterium NMR of specifically deuterated fluorine spin probes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't