Linked Life Data

7439542

Source:http://linkedlifedata.com/resource/pubmed/id/7439542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1981-2-26
pubmed:abstractText
The enhanced ability of liver to extract glucogenic precursors from plasma in insulinopenic diabetes was investigated at the cellular level by measuring amino acid transport in isolated hepatocytes from streptozotocin-diabetes rats. The concentrative Na+-dependent uptake of alpha-amino[1-14C]isobutyric acid (AIB), a non-metabolizable analog of alanine, was increased in hepatocytes from diabetic rats compared with controls. This increase was observed both at a low (0.1 mM) and high (30 mM) extracellular concentration of the amino acid. Analysis of the relationship between AIB influx and AIB concentration revealed that the increased uptake in diabetic rat hepatocytes was due to an enhanced capacity (i.e., a twofold increase in the Vmax) of the transport system whereas the apparent affinity (Km) for AIB was unaltered. The increased amino acid transport activity in diabetic rat hepatocytes was due mainly to an increased transport through system A (alanine-preferring) and, to a much lesser extent, through system ASC (alanine, serine, cysteine). System L (leucine-preferring) and AIB entry through simple diffusion were not affected in diabetic rat hepatocytes compared with controls. Increased AIB transport of diabetic rat hepatocytes was returned to normal by insulin treatment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0012-1797
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
996-1000
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Amino acid transport in isolated hepatocytes from streptozotocin-diabetic rats.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't