Linked Life Data

7439192

Source:http://linkedlifedata.com/resource/pubmed/id/7439192

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1981-2-19
pubmed:abstractText
The kinetics of the protonation changes and of the formation of the metarhodopsin II intermediate are measured in the presence of the pH indicator dye bromocresol purple after two consecutive flashes on the same suspension of right-side-out vesicles from cattle rod-outer-segment membranes. A rapid proton binding followed by partial proton release is observed. The formation of metarhodopsin II is found to be biphasic. The rapid phase of metarhodopsin II formation is slightly faster than the proton binding, and the kinetics of the slow phase are similar to that of the proton release. The protein groups which are protonated and deprotonated are shown to be situated on the outside of the vesicles. These results provide evidence that a structural change of the protein involving pK changes occurs without spectral modification before the formation of metarhodopsin III (half-time of the order of seconds).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-103
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Optical study of the light-induced protonation changes associated with the metarhodopson II intermediate in rod-outer-segment membranes.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't