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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1981-2-19
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pubmed:abstractText |
A complex containing deoxyribonucleic acid, protein and lipid has been isolated from the nuclei of the sea urchin Stronglocentrotus pupuratus by gentle lysis of nuclei and centrifugation of the lysate in a renografin gradient. The complex is similar in several respects to DNA-membrane complexes isolated by the sarkosyl M-band technique [1]. These include the following: 1, most of the cellular DNA is present in the complex but the bulk of the DNA may be removed by sonication or nuclease treatment; 2, nascent DNA is preferentially found in the complex; and 3, destruction of membrane/protein components removes DNA found in the complex.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0006-3002
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
17
|
pubmed:volume |
609
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
456-63
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7437435-Animals,
pubmed-meshheading:7437435-Cell Membrane,
pubmed-meshheading:7437435-Centrifugation, Density Gradient,
pubmed-meshheading:7437435-DNA Replication,
pubmed-meshheading:7437435-Diatrizoate Meglumine,
pubmed-meshheading:7437435-Embryo, Nonmammalian,
pubmed-meshheading:7437435-Membrane Lipids,
pubmed-meshheading:7437435-Membrane Proteins,
pubmed-meshheading:7437435-Nuclear Envelope,
pubmed-meshheading:7437435-Sea Urchins
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pubmed:year |
1980
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pubmed:articleTitle |
A potential DNA replication complex isolated from sea urchin embryos by renografin gradient centrifugation.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|