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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1979-4-25
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pubmed:abstractText |
A cholinesterase was partially purified from bush bean (Phaseolus vulgaris L.) roots by using acridinium-based ligand affinity chromatography. The procedure gave a 78-fold increase in specific activity, although at least three inactive contaminants remained. The enzyme activity was maximal against acetyl esters of choline and was inhibited by neostigmine. Di-isopropyl phosphorofluoridate completely inhibited activity at concentrations greater than 0.1 mM. The catalytic centre activity was 2 X 10(-4) times that of electric eel acetylcholinesterase. Cholinesterase activity appeared as a peak (s = 4.2 +/- 0.1 S) after isokinetic sedimentation. The Stokes radius was 4.00 nm and the apparent molecular weight was 72700 +/- 1900. The smallest active and native form of the enzyme appeared to be a monomer. This contrasts with animal acetylcholinesterases, in which the smallest active and native forms are multimeric.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-1125023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-13264563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-13726518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-13767412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-13936331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-14035715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-14240539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-14774389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-16591490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-16655776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-16658363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-16658656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-16658783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-17837670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-192194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-4115983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-4449474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-4515028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-4699232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-4824208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-4839042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-4842295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-4846184,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-844798,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/743233-976271
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
769-77
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pubmed:dateRevised |
2010-9-2
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pubmed:meshHeading |
pubmed-meshheading:743233-Binding Sites,
pubmed-meshheading:743233-Centrifugation, Density Gradient,
pubmed-meshheading:743233-Cholinesterases,
pubmed-meshheading:743233-Chromatography, Agarose,
pubmed-meshheading:743233-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:743233-Fabaceae,
pubmed-meshheading:743233-Kinetics,
pubmed-meshheading:743233-Molecular Weight,
pubmed-meshheading:743233-Plants,
pubmed-meshheading:743233-Plants, Medicinal,
pubmed-meshheading:743233-Substrate Specificity
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pubmed:year |
1978
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pubmed:articleTitle |
Partial purification and some properties of a cholinesterase from bush bean (Phaseolus vulgaris L.) roots.
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pubmed:publicationType |
Journal Article
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