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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2 Pt 1
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pubmed:dateCreated |
1981-1-26
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pubmed:abstractText |
The present study demonstrates the ability of plasma fibronectin or cold-insoluble globulin (Clg) to promote the uptake of 125I-labeled, gelatin-coated latex beads (g-Ltx*) by monolayers of peritoneal macrophages (PM). The uptake of g-Ltx* by PM was enhanced by Clg in a concentration-dependent fashion and required the presence of heparin (10 U/ml) as an obligatory cofactor for maximal particle uptake. Treatment of PM monolayers with trypsin (1 mg/ml) for 15 min at 37 degrees C after particle uptake removed less than 15% of the radioactivity incorporated by the monolayers. However, a similar trypsin treatment of the monolayers before the addition of latex particles depressed Clg-dependent uptake by greater than 75%. Pretreatment of PM monolayers with inhibitors of glycolysis effectively reduced the Clg-dependent uptake of latex. Similarly, pretreatment of monolayers with either inhibitors of protein synthesis or agents that disrupt cytoskeletal elements also significantly depressed Clg-dependent particle uptake. Phagocytosis of g-Ltx* by PM in the presence of Clg and heparin was confirmed by electron microscopy. Finally, g-Ltx* could also be effectively opsonized with Clg at 37 degrees C before their addition to the monolayers. These studies suggest that the recognition of g-Ltx* in the presence of Clg required cell surface protein(s) and that subsequent phagocytosis of these particles by PM was energy dependent and required intact intracellular cytoskeleton elements. Thus, PM monolayers provide a suitable system for further studies on the function of Clg in the recognition and phagocytosis of gelatin-coated particles by phagocytic cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-1011990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-1094920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-114206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-1263186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-13936765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-13940299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-13986422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-313445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-357987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-415138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-4356569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-4370051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-4565342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-4591850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-4704232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-4726862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-5033420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-5054338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-5055314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-5061735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-5113631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-5132788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-5884843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-69450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-71297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7430249-8645
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
427-33
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7430249-Animals,
pubmed-meshheading:7430249-Ascitic Fluid,
pubmed-meshheading:7430249-Colchicine,
pubmed-meshheading:7430249-Cycloheximide,
pubmed-meshheading:7430249-Cytochalasin B,
pubmed-meshheading:7430249-Fibronectins,
pubmed-meshheading:7430249-Gelatin,
pubmed-meshheading:7430249-Latex,
pubmed-meshheading:7430249-Macrophages,
pubmed-meshheading:7430249-Male,
pubmed-meshheading:7430249-Microspheres,
pubmed-meshheading:7430249-Phagocytosis,
pubmed-meshheading:7430249-Puromycin,
pubmed-meshheading:7430249-Rats
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pubmed:year |
1980
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pubmed:articleTitle |
Fibronectin-mediated uptake of gelatin-coated latex particles by peritoneal macrophages.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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