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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
21
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pubmed:dateCreated |
1981-1-29
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pubmed:abstractText |
ApoVLDL-II is a major apoprotein in avian very low density lipoproteins (Jackson, R. L., Lin, H.-Y., Chan, L., and Means, A.R. (1977) J. Biol. Chem. 252, 250-253). Partially purified apoVLDL-II mRNA was translated in vitro in a wheat germ system in the presence of various labeled amino acids. The product, designated preapoVLDL-II, was purified by immunoprecipitation and sodium dodecyl sulfate acrylamide gel electrophoresis. It was subjected to automated Edman degradation in a Beckman Sequencer. The signal peptide was found to be a 23-amino acid NH2-terminal extension of the mature protein with the following sequence: Met-Gln-Tyr-Arg-Ala-Leu-Val-Ile-Ala-Val-Ile-Leu-Leu-Leu-Ser-Thr-Val-Pro-Glu-Val-Cys-Ser-Lys where Lys is the NH2-terminal residue of mature apoVLDL-II. The abundance and distribution of the hydrophobic amino acid residues are very similar to those of other signal sequences and the average hydrophobicity for the 23 residues is -1227 cal/mol/residue. However, translocation of preapoVLDL-II would represent a unique case of vectorial migration of a protein through a membrane since apoVLDL-II is itself an apolipoprotein and binds lipid spontaneously.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
10
|
pubmed:volume |
255
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10060-3
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7430115-Amino Acid Sequence,
pubmed-meshheading:7430115-Animals,
pubmed-meshheading:7430115-Apolipoproteins,
pubmed-meshheading:7430115-Chickens,
pubmed-meshheading:7430115-Diethylstilbestrol,
pubmed-meshheading:7430115-Lipoproteins, VLDL,
pubmed-meshheading:7430115-Liver,
pubmed-meshheading:7430115-Male,
pubmed-meshheading:7430115-Plants,
pubmed-meshheading:7430115-Protein Biosynthesis,
pubmed-meshheading:7430115-RNA, Messenger,
pubmed-meshheading:7430115-Triticum
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pubmed:year |
1980
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pubmed:articleTitle |
Amino acid sequence of the signal peptide of apoVLDL-II, a major apoprotein in avian very low density lipoproteins.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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