Linked Life Data

7419529

Source:http://linkedlifedata.com/resource/pubmed/id/7419529

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1980-12-18
pubmed:abstractText
A novel method for the purification of poly(ADP-ribose) was established. Calf thymus nuclei were incubated with NAD. After pronase digestion, DNA was isolated as a flocculent form by adding 1 M NaCl and 2 volumes of ethanol. Acid-insoluble poly(ADP-ribose), which was synthesized during incubation, was quantitatively recovered in this DNA fraction. Poly(ADP-ribose) was purified as a single peak from a hydroxylapatite column after the nuclease treatment. Preparation of poly(ADP-ribose) thus obtained revealed the characteristics of pure poly(ADP-ribose), including a ratio of A280 to A260 of 0.24.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-924X
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
917-20
pubmed:dateRevised
2007-12-19
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
A novel method for purification of poly(ADP-ribose).
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't