Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1980-11-24
|
| pubmed:abstractText |
A procedure for the determinations of aprindine in human plasma was developed. After the addition of N,N-diethyl-N'-(1,2,3,4-tetrahydro-2-naphthyl)-N'-phenyl-1,3-propanediamine as an internal standard, the plasma was buffered to pH 8.0, and the drug and the internal standard were extracted into ethyl acetate-hexane (9:1 v/v). The compounds then were extracted from the organic phase into 0.02 N HCl. The acidic solution was made basic with 0.2 M tribasic sodium phosphate, and aprindine and the internal standard were extracted into a small volume of hexane. The compounds were analyzed by GLC using a nitrogen--phosphorus flame-ionization detector. The drug concentration and instrument response were linear for 0.10--1.00 microgram of aprindine/ml, the slope was 1.1416 (0.0141), the y intercept was 0.0096 +/- 0.0082, and the correlation coefficient was 0.99960 +/- 0.00002. The sensitivity of the method was 0.02 microgram of aprindine/ml. The within-day coefficient of variation was 9.50, 3.14, and 2.21% for 0.05, 0.20, 0.40, and 0.80 microgram of aprindine/ml, respectively. The between-day coefficient of variation was 17.4, 3.40, 2.07, and 1.54% at the same concentrations. Total precision values of 19.9, 4.60, 4.26, and 2.69% were obtained. The overall relative error of the method +1.33, +2.00, -0.07, and +0.25% at these concentrations.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-3549
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
69
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1094-6
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| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading | |
| pubmed:year |
1980
|
| pubmed:articleTitle |
GLC determination of aprindine in human plasma using a nitrogen--phosphorus flame-ionization detector.
|
| pubmed:publicationType |
Journal Article
|