Linked Life Data

7407071

Source:http://linkedlifedata.com/resource/pubmed/id/7407071

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1980-11-24
pubmed:abstractText
Both light-adapted and dark-adapted forms of bacteriorhodopsin in purple membrane in 67% glycerol solution were allowed to stand in acidic conditions by the addition of HCl to final concentrations from 4 X 10(-4) to 2 X 10(-2) M for 24 h at 3 degrees C. Over this concentration range, the acid-induced products from both species showed a maximum absorbance around 600 nm and high-performance liquid chromatography of extracted retinal isomers revealed that the acid-induced form of bacteriorhodopsin has 13-cis- and all-trans-retinals in a molar ratio of 4:6, which is intermediate between those of the dark-adapted and the light-adapted forms at neutral pH values. Exposure of the acid-induced form of bacteriorhodopsin to light at wavelengths longer than 670 nm at 3 degrees C caused a decrease of the absorbance around 600 nm with a concomitant rise of the absorbance around 500 nm. The extract from the irradiated products of bacteriorhodopsin in acid contained 9-cis- and 11-cis-retinals in addition to 13-cis- and all-trans-retinals. The absorbance maximum estimated from the analysis of the absorption spectra and the composition of the isomers was found at 495 nm for the 9-cis-retinal pigment and around 560 nm for the 11-cis-retinal pigment. On irradiation with 438-nm light, the 9-cis-retinal pigment disappeared with a concomitant increase of both the 13-cis- and all-trans-retinal pigments as judged by chromophore analysis and the absorption spectrum. The 9-cis-retinal pigment brought to pH 9 exhibited a maximum absorbance at 450 nm; this could be decomposed by the action of hydroxylamine or converted to a form resembling normal bacteriorhidopsin by 438-nm irradiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3825-31
pubmed:dateRevised
2001-11-2
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Formation of 9-cis- and 11-cis-retinal pigments from bacteriorhodopsin by irradiating purple membrane in acid.
pubmed:publicationType
Journal Article