Linked Life Data

7405594

Source:http://linkedlifedata.com/resource/pubmed/id/7405594

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1980-10-27
pubmed:abstractText
The effect of p-chloromercurybenzoate (p-CMB) on the MDH-activity of cytosol and submitochondrial fractions (matrix and inner membranes) of rat liver is studied in different buffer systems with identical pH. It is found that p-CMB has two effects on the MDH-activity, depending on the subcellular localization of the enzyme and on the type of buffer used: inhibitory (for the enzyme of the three fractions in HEPES-NaOH and potassium-phosphate buffers) and activating (for the enzyme of the submitochondrial fractions in Tris-HCI buffer). The effect of the SH-reagent on the formation and stability of enzyme-substrate complexes in the different buffers is also studied, as well as the temperature dependence of the p-CMB action on the MDH-activity of the preparations. It is found that the association of m-MDH with the inner mitochondrial membranes does not change its kinetic behaviour. The probable different conformation of the mitochondrial enzyme in the different buffer systems is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0323-9950
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
53-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Effect of p-chloromercurybenzoate on the MDH-activity of cytosol and rat liver submitochondrial fractions in different buffers.
pubmed:publicationType
Journal Article, In Vitro