Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1980-10-27
pubmed:abstractText
We have used quick-freezing and freeze-fracture to study early stages of exocytosis in rat peritoneal mast cells. Mast cells briefly stimulated with 48/80 (a synthetic polycation and well-known histamine-releasing agent) at 22 degrees C displayed single, narrow-necked pores (some as small as 0.05 micrometer in diameter) joining single granules with the plasma membrane. Pores that had become as large as 0.1 micrometer in diameter were clearly etchable and thus represented aqueous channels connecting the granule interior with the extracellular space. Granules exhibiting pores usually did not have wide areas of contact with the plasma membrane, and clearings of intramembrane particles, seen in chemically fixed mast cells undergoing exocytosis, were not present on either plasma or granule membranes. Fusion of interior granules later in the secretory process also appeared to involve pores; granules were often joined by one pore or a group of 2-4 pores. Also found were groups of extremely small, etchable pores on granule membranes that may represent the earliest aqueous communication between fusing granules.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-100501, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-1025155, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-1096303, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-1113505, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-11905197, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-13514685, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-183213, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-319098, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-334779, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-38256, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-4107023, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-4116105, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-4119437, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-4126372, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-4127384, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-4172484, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-4214825, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-4347978, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-4620378, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-4777235, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-5321660, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-559677, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-574515, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-582596, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-838774, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-856830, http://linkedlifedata.com/resource/pubmed/commentcorrection/7400221-885915
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
666-74
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Arrest of membrane fusion events in mast cells by quick-freezing.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.