Linked Life Data

7397205

Source:http://linkedlifedata.com/resource/pubmed/id/7397205

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1980-10-21
pubmed:abstractText
Liposomes containing either 32P-labeled diphosphatidylglycerol (cardiolipin) or 32P-labeled phosphatidyl-rac-(1)-glycerol were injected into the circulation of rats. Analysis of the liver lipids 2-3 after injection showed incorporation of the 32P label from both lipids to a lipid which had chromatographic properties identical with bis(monoacylglycero)phosphate. Stereochemical analysis of this lipid indicated that its backbone was sn-glycero-1-phospho-1'-glycerol. Cultured hamster fibroblasts (BHK cells) were incubated in a medium containing lyso[32P]phosphatidyl-rac-(1)-glycerol and the formation of radioactive lipids in the cells was followed. Bis(monoacylglycero) phosphate was the major 32P-labelled lipid formed: as much as 36.4% of the lyso[32P]phosphatidyl-rac-(1)-glycerol absorbed to the cells was converted to bis(monoacylglycero)phosphate. Similar results were obtained with lyso[32P]phosphatidyl-sn-1-glycerol as a precursor. Stereoanalysis of the bis(monoacylglycero)-[32P]-phosphate formed from either precursor indicated that this lipid was a derivative of sn-glycero-1-phospho-1'-glycerol. These results establish phosphatidylglycerol, diphosphatidylglycerol and lysophosphatidylglycerol as precursors of bis-(monoacyl-sn-glycero-1)phosphate in vivo. The mechanism of the conversion of lysophosphatidylglycerol to bis-(monoacyl-sn-glycero-1)phosphate was studied by using 32P,3H-labeled lysophosphatidyl-rac-(1)-glycerol as a precursor. Both labels were incorporated to bis(monoacylglycero)phosphate with similar efficiency, which suggests that rearrangement, rather than replacement, of the (originally acylated) sn-glycero-3-phospho moiety of the precursor is the essential reaction in the biosynthesis of the sn-glycero-1-phospho-1'-glycerol backbone of bis(monoacylglycero) phosphate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
618
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
407-19
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Conversion of phosphatidylglycerol lipids to bis(monoacylglycero)phosphate in vivo.
pubmed:publicationType
Journal Article