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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1980-9-23
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pubmed:abstractText |
Acute or chronic treatment of Sprague-Dawley rats with N-2-fluorenylacetamide (2-FAA) significantly increased the capacity of hepatic microsomes for N-hydroxylation of 2-FAA. The optimal increases, 11- and 16-fold, were measured in hepatic microsomes of male and female rats, respectively, 24 hr after a single intraperitoneal injection of 0.67 mmole 2-FAA/kg of body weight. The increases in 2-FAA N-hydroxylating activity were not paralleled by any appreciable changes in the content of cytochrome P-450. After oral intake of 2-FAA, small increases in the content of cytochrome b5 and of total heme were measured. Gel electrophoresis showed the presence of a new protein, staining for heme, in the MW region of 46,000 as soon as 24 hr after treatment of rats with at least 0.34 mmole 2-FAA/kg of body weight. The intensity of this protein band was enhanced by prolonged treatment of rats with 2-FAA, but was not paralleled by the extent of induction of N-hydroxylation of 2-FAA. This protein was induced in hepatic microsomes of rats both susceptible (male) and nonsusceptible (female) to hepatocarcinogenesis by 2-FAA. The data suggest that the 2-FAA-induced protein may not be a specific marker of early preneoplastic changes in rat liver.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Acetylaminofluorene,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes,
http://linkedlifedata.com/resource/pubmed/chemical/Heme,
http://linkedlifedata.com/resource/pubmed/chemical/Hemeproteins
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0034-5164
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-64
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7394317-2-Acetylaminofluorene,
pubmed-meshheading:7394317-Animals,
pubmed-meshheading:7394317-Cytochrome P-450 Enzyme System,
pubmed-meshheading:7394317-Cytochromes,
pubmed-meshheading:7394317-Female,
pubmed-meshheading:7394317-Heme,
pubmed-meshheading:7394317-Hemeproteins,
pubmed-meshheading:7394317-Hydroxylation,
pubmed-meshheading:7394317-Liver Neoplasms,
pubmed-meshheading:7394317-Male,
pubmed-meshheading:7394317-Microsomes, Liver,
pubmed-meshheading:7394317-Neoplasms, Experimental,
pubmed-meshheading:7394317-Rats,
pubmed-meshheading:7394317-Time Factors
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pubmed:year |
1980
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pubmed:articleTitle |
Effects of acute or chronic treatment of rats with N-2-fluorenylacetamide on its N-hydroxylation and on hemoproteins of hepatic microsomes.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|