Linked Life Data

7392047

Source:http://linkedlifedata.com/resource/pubmed/id/7392047

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1980-9-23
pubmed:abstractText
The necessity of low temperature preparative procedures for diffusible ion localization using the ion microscope has been established with rat liver as the test material. Using conventional fixation procedures, significant ion loss and redistribution were observed which exceeded the 1 micrometer lateral resolution of the direct imaging ion microanalyser. The compositional morphology evidenced in chemically prepared versus fast-frozen, freeze-dried, unfixed material was compared and evaluated in terms of ion retention, ion redistribution and morphological integrity. A mass spectral analysis of both preparations has been presented and discussed with regard to spectral interferences and elemental sensitivities. Ion images of sodium and potassium were recorded for chemically prepared liver sections and compared with the ion distributions obtained for unfixed, fast-frozen, freeze-dried 1 micrometer thick sections. The magnitude of ion loss during preparation of samples removed at different stages in the course of chemical processing was determined by atomic absorption and flame emission spectrophotometry.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-2720
pubmed:author
pubmed:issnType
Print
pubmed:volume
118
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
409-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Diffusible ion localization by ion microscopy: a comparison of chemically prepared and fast-frozen, freeze-dried, unfixed liver sections.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.