Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1980-9-28
|
| pubmed:abstractText |
A sensitive and specific high-performance liquid chromatographic method was developed for the determination of ketoprofen [2-(3-benzoylphenyl)propionic acid] in plasma and urine. The method includes an extraction of the drug and the internal standard [2-(4-benzoylphenyl)butyric acid] into ether from acidified plasma. The organic phase is evaporated, and the residue is dissolved in the mobile phase (acetonitrile-0.02 M phosphate buffer, pH 3) (45:55). A 20-microliter aliquot is analyzed on a reversed-phase column. The accuracy is within 1.5% for therapeutic concentrations, and the coefficients of variation are 5.5 and 3.4% for 2 and 10 micrograms/ml, respectively. For the urine assay, the accuracy is within 3%, and the cofficients of variation are 1.9 and 1.7% for 3 and 50 micrograms/ml, respectively. This method was applied successfully to the determination of ketoprofen in humans for pharmacokinetic studies.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0022-3549
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
69
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
763-5
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading | |
| pubmed:year |
1980
|
| pubmed:articleTitle |
Determination of ketoprofen in biological fluids by reversed-phase chromatography.
|
| pubmed:publicationType |
Journal Article
|