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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1980-8-15
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pubmed:abstractText |
Cells of two teratocarcinoma stem cell lines (PCC4 azaguanine [aza] 1 and F9 5-bromodeoxyuridine [BrdU]) were fused with normal mouse spleen cells and mouse thymoma-derived cells (BW 5147), respectively. Hybrid clones were tested for the expression of molecules coded by the H-2K and -2D genes both by absorption analysis of conventional H-2 sera and by indirect antibody-binding radioimmunoassay with monoclonal antibodies. Somatic cell hybrids between PCC4 aza 1 and spleen cells morphologically resemble teratocarcinoma stem cells and do not express H-2 antigens. However, after differentiation in vitro, one of these hybrid clones expresses the H-2K and -2D gene products of both parental cell lines, one close expresses H-2-D- but not H-2K-coded antigenic determinants, and one clone remains H-2 negative. Somatic cell hybrids between F9 BrdU and BW 5147 resemble fibroblasts. Analysis of a series of hybrid clones revealed some clones that express both the H-2K- and H-2D-coded antigenic specificities of both parental alleles, some that express H-2D gene products strongly and the H-2K gene products very weakly, and some that express H-2D- but not H-2K-coded molecules. These results imply independent regulation of expression of the H-2K and -2D genes. The H-2D gene products appear to be preferentially expressed if the hybrid cells are capable of expressing H-2. The results suggest complex regulatory mechanisms that are H-2K and H-2D specific.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-122984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-14168277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-358801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-396115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-398326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-4544550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-4599372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-5436899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-558524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-567555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-605381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-61803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-61820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-87443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7381363-89977
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
151
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1349-59
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7381363-Animals,
pubmed-meshheading:7381363-Antibody Specificity,
pubmed-meshheading:7381363-Cell Differentiation,
pubmed-meshheading:7381363-Clone Cells,
pubmed-meshheading:7381363-Genes,
pubmed-meshheading:7381363-H-2 Antigens,
pubmed-meshheading:7381363-Hybrid Cells,
pubmed-meshheading:7381363-Isoantibodies,
pubmed-meshheading:7381363-Major Histocompatibility Complex,
pubmed-meshheading:7381363-Mice,
pubmed-meshheading:7381363-Neoplasms, Experimental,
pubmed-meshheading:7381363-Spleen,
pubmed-meshheading:7381363-Teratoma
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pubmed:year |
1980
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pubmed:articleTitle |
Independent regulation of H-2K and H-2D gene expression in murine teratocarcinoma somatic cell hybrids.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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