Linked Life Data

7380840

Source:http://linkedlifedata.com/resource/pubmed/id/7380840

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1980-8-28
pubmed:abstractText
The mRNA coding for pre-prolactin (pPRL) of the adult bovine anterior pituitary was purified to 85% homogeneity and used as a template for cDNA synthesis with reverse transcriptase from avian myeloblastosis virus. The cDNA sequences complementary to pPRL mRNA were further purified by employing a limited back-hybridization step and treatment with S1 nuclease. The pPRL cDNA preparation was judged to be homogeneous by comparing its hybridization kinetics to those of ovalbumin and globin mRNA standards and by thermal melt analysis of the pPRL mRNA:cDNA hybrids. Total cellular RNA was extracted from individual bovine fetal pituitaries of either sex, ranging from 90 to 200 days of gestation, and examined for its pPRL mRNA concentration by hybridization with an excess of pPRL cDNA. The hybridization assay was capable of detecting picogram amounts of pPRL mRNA, e.g. amounts less than 0.002% of input total cellular RNA. These results indicated that from 90 to 200 days of gestation, the levels of pPRL mRNA relative to total cellular RNA in bovine fetal pituitaries increase exponentially. This increase in pPRL mRNA occurs to the same extent in either sex, with the most dramatic shift (over 10-fold) occurring between 120 and 145 days of gestation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
255
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5871-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Ontogeny of pituitary hormone mRNAs in the bovine fetus. Quantitation of pre-prolactin mRNA as a function of gestation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.