Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1980-8-15
pubmed:abstractText
The action spectrum and quantum efficiency (phi H+) for proton ejection from Halobacterium halobium have been determined under conditions chosen to minimize light-triggered proton influx which is usually observed in intact cells. The action spectrum for the carotenoid-containing strain, R1, shows that light energy absorbed by the carotenoids does not contribute to the proton ejection. After correction for shielding by the carotenoids and other cell pigments, the action spectrum closely follows the absorption spectrum of bacteriorhosopsin. Values determined for phi H+ in H. halobium cells and cell envelopes range from 0.4 to 0.7. These values are significantly higher than the currently accepted value for the quantum efficiency for the photoreaction cycle of bacteriorhodopsin in isolated purple membrane, suggesting that at least in intact cells and envelopes more than one proton is pumped during the bacteriorhodopsin photocycle. A new nondestructive assay for bacteriorhopopsin in intact cells and envelopes which also contain other pigments was used in this work.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2152-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Action spectrum and quantum efficiency for proton pumping in Halobacterium halobium.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.