Linked Life Data

7378353

Source:http://linkedlifedata.com/resource/pubmed/id/7378353

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1980-8-15
pubmed:abstractText
The tryptophan content of purified bovine rhodopsin was obtained by two independent methods: direct analysis of hydrolysates prepared by digestion of opsin with methanesulfonic acid containing 0.2% 3-(2-aminoethyl)indole and a computer-assisted analysis of the near-UV spectrum of rhodopsin. Both methods gave a value of eight tryptophan residues per rhodopsin. Based on the near-UV spectral analysis, the light-induced difference spectrum of rhodopsin, and the susceptibility of residues to oxidation by N-bromosuccinimide, we concluded that approximately half of the tyrosine and tryptophan residues are shielded to some extent from the aqueous solvent, that two of the tryptophan residues are in very apolar environments, and that following light excitation at least one of these tryptophan residues and several tyrosines are exposed to an aqueous environment. Analysis of rhodopsin absorption in the far-UV indicated that below 240 nm, approximately half of the absorption is due to aromatic residues and that the other half is largely due to the peptide bond. The effect of illumination on secondary structure is to induce a loss in helical structure, calculated to involve 35% of the amino acid residues in purified rhodopsin. If light-induced changes in secondary structure are specifically excluded, most of these results can be extended to bovine rod outer segment membranes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2145-51
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Tryptophan in bovine rhodopsin: its content, spectral properties and environment.
pubmed:publicationType
Journal Article