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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1980-7-28
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pubmed:abstractText |
The addition of cholic acid to growing cultures of Eubacterium species V.P.I. 12708 caused a 25 and 46-fold increase in 7 alpha-dehydroxylation activity using cell extracts or whole cell suspensions, respectively. Bile acid conversion rates using either [14C]-cholic acid or [14C]-chenodeoxycholic acid as substrates increased at approximately the same rate when either cholic or chenodeoxycholic acid was added to growing cultures as inducer. The induction of 7 alpha-dehydroxylase activity was highly specific requiring a free C-24-carboxyl group and an unhindered 7 alpha-hydroxy group on the B ring of the steroid nucleus. Unexpectedly, cholic acid also rapidly induced NADH:flavin oxidoreductase activity in growing cultures of this bacterium.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0039-128X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
103-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7376208-Bile Acids and Salts,
pubmed-meshheading:7376208-Cholic Acids,
pubmed-meshheading:7376208-Colonic Neoplasms,
pubmed-meshheading:7376208-Enzyme Induction,
pubmed-meshheading:7376208-Eubacterium,
pubmed-meshheading:7376208-Feces,
pubmed-meshheading:7376208-Humans,
pubmed-meshheading:7376208-Hydroxysteroid Dehydrogenases,
pubmed-meshheading:7376208-Kinetics,
pubmed-meshheading:7376208-Oxidoreductases,
pubmed-meshheading:7376208-Steroid Hydroxylases,
pubmed-meshheading:7376208-Structure-Activity Relationship
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pubmed:year |
1980
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pubmed:articleTitle |
Bile acid induction specificity of 7 alpha-dehydroxylase activity in an intestinal Eubacterium species.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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