Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1980-6-25
|
| pubmed:abstractText |
We describe the rapid, inexpensive fluorometry of hemoglobin in undiluted whole blood. The procedure consists only of adding a standard quantity of a fluorescent dye to a measured volume (approximately 15 microL) of blood, together with some solubilizing detergent. The assay is based on the attenuation of the dye's fluorescence (excited within the region 400--440 nm) that results from the competitive absorption of exciting light by the hemoglobin present--the "inner filter effect." The wavelength that one can use is optional and will determine which dyes can be used. Measurements are made with the hematofluorometer, a "front-face" filter fluorometer (Blumberg et al., J. Lab. Clin. Med. 89: 712--723, 1977). We demonstrate the validity of the method for two dyes, rhodamine B and fluorescein dibutyrate, which we used with hematofluorometers that were designed to determine blood zinc protoporphyrin and bilirubin, respectively. Our method exhibited a standard error of about 4 g of hemoglobin per liter vs the comparison method (Coulter Counter method, for which the CV is 1.2%). The CV is about 3%. The method seems appropriate for "field" use (i.e., use outside the laboratory) in anemia-screening programs.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0009-9147
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
409-13
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1980
|
| pubmed:articleTitle |
Hemoglobin determined in 15 microL of whole blood by "front-face" fluorometry.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|