Linked Life Data

73561

Source:http://linkedlifedata.com/resource/pubmed/id/73561

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1978-2-18
pubmed:abstractText
Classical measurements of cytotoxicity using dye exclusion and microscopic evaluation are both time-consuming and inaccurate. Using a cell sorter (TPS) a single dye system has been developed which stains live and complement killed cells with different fluorescence intensity. After exposure of target cells to antibody and complement, ethidium bromide is added to the target cells at a high enough concentration to stain complement killed cells very intensely. The cells are then diluted and lysed to produce single nuclei, permitting live cells to be stained but less intensely than the dead cells. Fluorescence intensity is measured on single nuclei at a rate of 10,000 per minute. For these studies anti-T antisera was titrated for complement dependent cytotoxic activity using normal mouse spleen cells, spleen cells from an anti-thymocyte serum treated mouse, and nylon wool purified mouse splenic T-cells. This procedure makes possible, reliable and reproducible measurement of cytotoxic activity on 5,000 cells per determination.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-16
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Automated fluorescent analysis for cytotoxicity assays.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.