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pubmed:abstractText |
Repeated washing of the particulate material from rat striatum abolishes the dopamine-sensitivity of the adenylate cyclase activity. Readdition of the soluble fraction of the caudate homogenate restores the dopamine-sensitivity to the enzyme activity. Fractions, prepared with thin layer chromatography, containing endogenous GTP and GDP also restore dopamine-sensitivity to striatal adenylate cyclase activity. The effectiveness of GDP results from its conversion to GTP during the assay; when this conversion is eliminated, GDP can specifically block the coupling between the dopamine receptor and adenylate cyclase.
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