Linked Life Data

7346382

Source:http://linkedlifedata.com/resource/pubmed/id/7346382

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1982-10-21
pubmed:abstractText
Labelled phosphatidylglycerol was incubated with rat liver lysosomes from animals treated for 3 to 20 days with chloroquine diphosphate. The longer the period of pretreatment with the amphiphilic drug, the greater was the increase in the synthesis rate of bis(monoacylglycero)phosphate, both in the absolute values and when related to the lysosomal protein which was also increased. The mechanism of the in vitro conversion of phosphatidylglycerol to bis(monoacylglycero)phosphate was studied by using phosphatidylglycerol labelled with 14C and/or 3H in different positions of the molecule. Assays with rac-1-(1,2-diacyl-[2-3H]glycero-3-phospho)-[U-14C]glycerol clearly demonstrated that the 3H/14C ratio of the substrate was the same as found in the product bis(monoacylglycero)phosphate. Therefore the whole glycerophosphoglycerol backbone of the substrate is used for bis(monoacylglycero)phosphate formation, and recombination of released glycerol moieties can be excluded. Experiments with phosphatidylglycerol labelled in both fatty acids suggest that only one acyl group of the substrate is preserved in bis(monoacylglycero)-phosphate. The analysis of further products formed during incubations of rat liver lysosomes with labelled phosphatidylglycerol showed a rapid degradation of the glycerolipid mainly by the action of phospholipase A and C.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0018-4888
pubmed:author
pubmed:issnType
Print
pubmed:volume
362
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1229-36
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Production of bis(monoacylglycero)phosphate from phosphatidylglycerol in isolated liver lysosomes of chloroquine-pretreated rats.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't