Linked Life Data

7341288

Source:http://linkedlifedata.com/resource/pubmed/id/7341288

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1982-7-8
pubmed:abstractText
Microgranular DEAE-cellulose anion exchanger with exchange capacities ranging between 0-2.7 meq/gr dry materials were tested for their suitability to be used as microcarriers for culturing several anchorage-dependent-cells. It was found that cells from established cell lines (BHK, MDCK), grew as confluent monolayer on DEAE-cellulose microcarriers with exchange capacity ranging between 1.00-2.0 meq/gr dry materials. Primary cells (chick embryo fibroblast) grow as a cell-microcarriers aggregate on DEAE-cellulose microcarriers with an exchange capacity ranging between 1.59-2.0 meq/gr dry materials. Cells from human diploid cell strains grew poorly on these microcarriers. Cell yields obtained using the DEAE-cellulose microcarriers were similar to those obtained on commercially available DEAE-dextran microcarrier culturing systems. Several viruses were propagated on cells grown in DEAE-cellulose microcarrier culturing systems. Virus yields per cell were similar to those obtained in conventional monolayer cultures.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-5149
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
115-23
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Cell and virus propagation on cylindrical cellulose based microcarriers.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't