Linked Life Data

7338507

Source:http://linkedlifedata.com/resource/pubmed/id/7338507

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1982-6-21
pubmed:abstractText
1. Membrane fraction containing halorhodopsin was prepared from the lysate of a mutant strain of Halobacterium halobium, Y1, which is defective in bacteriorhodopsin synthesis. 2. Irradiation of the membrane with red light at 0 degrees C decreased the absorbance intensities over the whole range of 500--600 nm and a new absorption peak appeared at about 400 nm. This process was reversed either by irradiation with blue light or simply by incubation in the dark. The wavelength at which the red light-induced absorbance decrease became maximum was 566 nm in the absence of NaCl and moved to 576 nm upon addition of NaCl. The midpoint for the conversion was at about 0.1 M NaCl. 3. Even though the membrane containing halorhodopsin was prepared in the absence of NaCl, this pigment was photochemically active in the sense that it could form a bathochromic photoproduct, a batho-intermediate, at -196 degrees C. 4. Retinal isomer composition during irradiation with red light at 0 degrees C was determined. Unirradiated halorhodopsin had predominantly all-trans retinal. With the increase of the blue-shifted product on irradiation, 13-cis content increased.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-924X
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1267-73
pubmed:dateRevised
2007-12-19
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Light-induced reaction of halorhodopsin prepared under low salt conditions.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't