Linked Life Data

7338126

Source:http://linkedlifedata.com/resource/pubmed/id/7338126

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1982-6-14
pubmed:abstractText
A method is presented for preparing conventionally frozen biopsy material of thyroid tissue for flow cytometric measurement of DNA content in individual cells. The accuracy of the method was established by measuring cell suspensions of 24 biopsies of normal thyroid tissue from 20 patients. Biopsy material immersed in Histocon preservative solution prior to freezing was found to give better results than material frozen dry. All samples were found to have approximately the same modal DNA value (i.e. diploid), as determined by calibration of the fluorescence measurement with a standard of fluorescent microspheres. It was found that for an unknown sample, a deviation in modal DNA content of more than 13-14 per cent from diploid can be classified as significant by this technique.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0272-7749
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Cellular DNA content in normal human thyroid: a flow cytometric study based on frozen biopsy material.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't