Linked Life Data

7335102

Source:http://linkedlifedata.com/resource/pubmed/id/7335102

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-5-21
pubmed:abstractText
Sulphur mustard (SM) (5 x 10(-8) M) given to primary Syrian hamster fibroblasts growing short-term in vitro produces a very sharp peak of chromatid aberrations 12-16 h after treatment. The composition of this peak has been investigated in relation to the cell cycle using the facility to divide S-phase into 5 cytologically defined sub-phases on the basis of replication band patterns following bromodeoxyuridine incorporation. It is shown that: (1) Considerable delay and perturbation of the cycle is present. (2) A major contribution to the aberration peak comes from pre-S cells, and the highest aberration frequencies are observed in such cells. (3) The bulk of the contribution from S-cells comes from the last subphase, SkV. (4) The majority of early S cells (sub-phases SkI-IV) fail to reach division within 36 h. Of the total S-cells scored, 54% are non-SkV in controls but only 14% after SM. (5) Aberrations are localized to late-replicating regions in SkV but are random in pre-S. (6) No measurable perturbation of replication programme was found in chromosome arms synthesizing in late SkV after SM. (7) The rapid fall in aberration frequency at later sampling times is consistent with a quick and efficient repair of DNA lesions which is known to occur after SM alkylation. The preferential loss of early S cells seems most likely to result from selective lethality, though differential delay and perturbation may make a contribution. It is interesting to note that the subphases missing are just those where the euchromatin replicates (early replicating R and G bands). The late-replicating chromatin, some of which is known to be dispensable in Syrian hamster, is confined to SkV, the sub-phase which appears to survive this dose of SM.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
375-87
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Differential effects of sulphur mustard on S-phase cells of primary fibroblast cultures from Syrian hamsters.
pubmed:publicationType
Journal Article