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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1982-5-27
|
| pubmed:abstractText |
Laser photon correlation spectroscopy and analytical ultracentrifugation were used to compare the equilibrium properties of disassembled, assembly-competent, brain microtubule protein from dogfish (Squalus acanthus) and beef. Analytical ultracentrifugation confirmed that assembly-competent bovine material at 4 degrees C, purified in the presence of glycerol through 2 cycles of assembly and disassembly, consisted of 6 S (dimer) and 35 S (ring) components, whereas assembly-competent dogfish material prepared in the same way was composed primarily of 6 S protein. By means of photon correlation spectroscopy, z-average diffusion coefficients (D'20, w) of 0.55 (+/- 0.04) x 10(-11) m2 s-1 (beef) and 1.27 (+/- 0.11) x 10(-11) m2 s-1 (dogfish) were measured at 4 degrees C for the twice-cycled assembly competent microtubule protein. Although D'20, w values for dogfish material are significantly higher than those for beef, they depart from the calculated value of 5.0 x 10(-11) m2 s-1 for a pure 6 S (dimer) solution. Microtubule accessory proteins were detectable on sodium dodecyl sulphate/polyacrylamide gels of dogfish microtubule protein. By fitting photocount autocorrelation functions to a 2-particle (dimer-ring) model it was estimated that the presence in a solution of assembly-competent dogfish microtubule protein of less than 1 ring per 250 dimers could account for the measured z-average diffusion coefficient. The temperature sensitivity of D'20, w showed that dogfish brain microtubule protein polymerized at lower temperatures than samples prepared by the same methods from beef brain.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0021-9533
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
52
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
137-49
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| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7334054-Animals,
pubmed-meshheading:7334054-Brain Chemistry,
pubmed-meshheading:7334054-Cattle,
pubmed-meshheading:7334054-Centrifugation, Density Gradient,
pubmed-meshheading:7334054-Diffusion,
pubmed-meshheading:7334054-Dogfish,
pubmed-meshheading:7334054-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7334054-Lasers,
pubmed-meshheading:7334054-Scattering, Radiation,
pubmed-meshheading:7334054-Temperature,
pubmed-meshheading:7334054-Tubulin
|
| pubmed:year |
1981
|
| pubmed:articleTitle |
Laser-light scattering diffusion measurements on brain microtubule protein from dogfish (Squalus acanthus) and beef.
|
| pubmed:publicationType |
Journal Article
|