Linked Life Data

7327599

Source:http://linkedlifedata.com/resource/pubmed/id/7327599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1982-4-12
pubmed:abstractText
Smooth muscle cells (SMC) were cultured from atherosclerotic plaques and uninvolved arteries to determine if differences exist between growth characteristics or ultrastructure of the cultured cells. Eighteen aortic punch biopsies provided the uninvolved tissue, and 58 carotid plaques provided the atherosclerotic tissue. Eighty percent of the samples yielded viable cultured cells, which reached a maximum population doubling time during log phase growth of 72 h (seeding density = 1.0 x 10(4) cells/cm2, 2nd passage). Growth characteristics of both normal and plaque-derived cells were the same in vitro. Growth rate declined with time in culture, and cell division ceased by the 5th or 6th passage. In culture, spindle shaped cells formed the "hill and valley" configuration typical of SMC. Plaque-derived SMC were ultrastructurally similar to SMC from uninvolved vessel wall. Proliferative potential did not vary with age or sex, with method of culture, or with whether the cells were plaque derived or not.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0073-5655
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
713-8
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Human smooth muscle cells cultured from atherosclerotic plaques and uninvolved vessel wall.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.