Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-3-13
pubmed:abstractText
1. Two proteodermatan sulphate fractions (I and II) from bovine sclera were studied by gel chromatography, light-scattering and ultracentrifugation under various conditions. 2. Gel chromatography of proteoglycans in the absence or presence of hyaluronate was performed under associative conditions. No effect on the elution profile was noted. 3. Ultracentrifugation experiments (sedimentation-velocity and sedimentation-equilibrium) with proteoglycan I and II in 6 M-guanidine hydrochloride gave molecular weights (Mw) of 160000-220000 and 70000-100000 respectively. As the protein contents were 45% and 60% respectively, it may be calculated that proteoglycan I contained four to five side chains, whereas proteoglycan II contained one or two. Sedimentation-equilibrium runs performed in 0.15 M-NaCl gave an apparent molecular weight (Mw) of 500000-800000 for proteoglycan I and 90000-110000 for proteoglycan II. 4. In light-scattering experiments both proteoglycans I and II yielded high particle weights in 0.15 M-NaCl (3.1 X 10(6) and 3.4 X 10(6) daltons respectively). In the presence of 6 M-guanidine hydrochloride the molecular weights decreased to 410000 and 130000 respectively. The particle weights in 0.15 M-NaCl were not altered by the addition of hyaluronate or hyaluronate oligosaccharides. 5. The dermatan sulphate side chains of scleral proteoglycans (L-iduronate/D-glucuronate ratio 7:13) gave a particle weight of 100000 daltons in 0.15 M-NaCl. In 1.00 M-KCl/0.02M-EDTA the molecular weight was 24000. Addition of free scleral dermatan sulphate chains to a solution of proteoglycan II promoted further multimerization of the macromolecule.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-1122296, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-125595, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-127551, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-13971270, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-14155091, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-194895, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-216413, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-220958, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-4222033, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-4236454, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-4263642, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-4273187, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-4277351, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-4277352, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-4277512, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-646789, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-7305918, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-762160, http://linkedlifedata.com/resource/pubmed/commentcorrection/7325967-949499
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
197
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
483-90
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Self-association of dermatan sulphate proteoglycans from bovine sclera.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't