Linked Life Data

7325454

Source:http://linkedlifedata.com/resource/pubmed/id/7325454

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1982-3-26
pubmed:abstractText
Capsular antigens were isolated from Pasteurella multocida, strain P-1059 and their immunogenicity was tested in turkeys. The crude capsular antigen (CCA) was extracted from bacterial cells grown on membranes by heating at 56 C in a 2.5% NaCl solution. The purified polysaccharide antigen (PPA) was obtained by precipitation of CCA by cetylpyridinium chloride. Young adult turkeys were inoculated at 0 and 14 days and were challenge exposed at 28 days by IM inoculation of a live culture of P-1059. The turkeys were observed for 2 weeks and mortality was recorded; bacterial isolation was done at the time of necropsy. In 3 trials, CCA provided 80% to 100% protection; in 1 trial, PPA failed to provide protection. Freund's incomplete adjuvant and aluminum hydroxide gel were effective as potentiating agents when higher than 280 microgram of CCA was used. The CCA showed significant (P less than 0.05) protection after treatment with heat (100 C, 5 min), chloroform, or trypsin, but lost its immunogenicity completely by acid hydrolysis. The CCA was not toxic to mice at 2 mg. The limulus lysate test showed that CCA contained endotoxin in less than or equal to 5% of the total solids. These results indicate that the surface antigen isolated from P multocida by saline extraction was immunogenic in young adult turkeys.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0002-9645
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1838-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Immunogenicity of capsular antigens of Pasteurella multocida in turkeys.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't