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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1976-10-1
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pubmed:abstractText |
When envelope-free spinach chloroplasts are incubated with stromal protein, catalytic NADP, catalytic ADP, radioactive bicarbonate and fructose 1,6-bisphosphate, 14CO2 fixation starts immediately upon illumination but oxygen evolution is delayed. The delay is increased by the addition of fructose 6-phosphate and by a variety of factors known (or believed) to increase fructose bisphosphatase activity (such as dithiothreitol, more alkaline pH, higher [Mg] and antimycin A). Conversely, the lag can be decreased or eliminated by the addition of an ATP-generating system. Bearing in mind the known inhibition, by ADP, of sn-phospho-3-glycerate (3-phosphoglycerate) reduction it is concluded that the lag in O2 evolution results from the production of ribulose 5-phosphate from fructose bisphosphate and that this in turn inhibits the reoxidation of NADPH by adversely affecting the ADP/ATP ratio. The results are discussed in their relation to the mode of action of antimycin A and to regulation of the reductive pentose phosphate pathway.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/Dithiothreitol,
http://linkedlifedata.com/resource/pubmed/chemical/Fructose-Bisphosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Fructosephosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Hexosediphosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
440
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
147-62
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pubmed:dateRevised |
2004-8-10
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pubmed:meshHeading |
pubmed-meshheading:7321-Carbon Dioxide,
pubmed-meshheading:7321-Chloroplasts,
pubmed-meshheading:7321-Dithiothreitol,
pubmed-meshheading:7321-Ethics Committees, Research,
pubmed-meshheading:7321-Fructose-Bisphosphatase,
pubmed-meshheading:7321-Fructosephosphates,
pubmed-meshheading:7321-Hexosediphosphates,
pubmed-meshheading:7321-Hydrogen-Ion Concentration,
pubmed-meshheading:7321-Magnesium,
pubmed-meshheading:7321-Oxygen,
pubmed-meshheading:7321-Oxygen Consumption,
pubmed-meshheading:7321-Photosynthesis,
pubmed-meshheading:7321-Plants
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pubmed:year |
1976
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pubmed:articleTitle |
Photosynthesis in a reconstituted chloroplast system from spinach. Some factors affecting CO2-dependent oxygen evolution with fructose-1,6-bisphosphate as substrate.
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pubmed:publicationType |
Journal Article
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