Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1982-3-22
pubmed:abstractText
1. Rapid inward Na current (INa) was studied in isolated cells from rat ventricular myocardium by a double-suction-pipette voltage clamp technique. All experiments were carried out at 20-22 degrees C. 2. INa elicited by single depolarizing voltage steps from a holding potential, VH, of -80 mV had a threshold between -70 and -60 mV and was maximal at -30 to -20 mV. Peak currents in Krebs-Ringer solution containing 145 mM Na were of the order 0.9-1.8 mA cm-2, assuming an average cell surface area of 8000 square micrometers. 3. The reversal potential for INa was predicted by the Nernst equation for external Na in the range 1.45-145 mM with 16 mM-Na solution perfusing the interior of the cell. 4. Instantaneous I-V plots were linear for potentials of -100 to + 10 mV. Maximum Na conductance (-gNa) was calculated to be 25 mS cm-2 in 145 mM-Na solutions and gNa was constant for potentials positive to -10 mV. 5. INa activated with a time constant of 0.7 msec at -55 mV, decreasing to 100 microsec on depolarizations positive to + 10 mV. 6. Two time constants (tau h1, tau h2) were required to describe INa inactivation during a maintained depolarization, with tau h2 three to four times as long as tau h1. tau h1 was about 2 msec at -50 mV, decreasing to 0.9 msec at -10 mV. 7. The time course for recovery of INa from inactivation also exhibited two time constants (tau r1, tau r2), with the longer tau r2 having a maximum value of the order 100 msec in the potential range -60 to -80 mV. 8. INa in isolated rat cardiac cells has a low sensitivity to tetrodotoxin, requiring a concentration of 30 micrometers for complete blockade.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-1013286, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-14946713, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-14946714, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-14946715, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-201723, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-304888, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-4153804, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-423977, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-423978, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-4415790, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-5016037, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-5101235, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-5234193, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-5241427, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-529142, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-5460932, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-5501485, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-5503866, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-571107, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-591911, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-591921, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-5972158, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-6251204, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-6255144, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-660159, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-674687, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-7320901, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-7381428, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-7381760, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-859585, http://linkedlifedata.com/resource/pubmed/commentcorrection/7320902-958493
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:volume
318
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
479-500
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Sodium current in single rat heart muscle cells.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't