Linked Life Data

7317353

Source:http://linkedlifedata.com/resource/pubmed/id/7317353

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1982-3-13
pubmed:abstractText
The transformation of glucocorticoid--receptor complex in the cytosol from lactating mouse mammary tissue was studied by using elevated temperature and KCl as promoters of the transformation reaction. The transformed receptor was identified from the nontransformed receptor by the following criteria: (a) increased binding to DNA--cellulose, (b) increased binding to ATP--Sepharose, (c) higher affinity for the steroid as determined by steroid dissociation kinetics, and (d) different sedimentation profiles on sucrose gradients containing KCl and sodium molybdate. A greater percentage of the nontransformed receptor was converted to the transformed state by an increased KCl concentration as opposed to increased temperature. Pretreatment of cytosol with 10 mM sodium molybdate prevented both the temperature- and salt-mediated transformation of the receptor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6790-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Transformation of mammary cytoplasmic glucocorticoid receptor under cell-free conditions.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't