Linked Life Data

7313549

Source:http://linkedlifedata.com/resource/pubmed/id/7313549

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1982-2-22
pubmed:abstractText
Cell lines that secreted antibodies to the hapten azobenzenearsonate (ABA) were established by hybridization of immune A/J spleen cells to the non-secreting myeloma, NS-1. Solid-phase radioimmunoassays (RIA) were developed for rapid screening of hybridoma supernatants to detect antibodies to ABA and to detect antibodies bearing the ABA cross-reactive idiotype (CRI). Hybrid clones secreting both CRI+ and CRI-- anti-ABA antibodies were obtained. The supernatant from one clone (7-13) strongly inhibited binding of iodinated anti-idiotype serum in a competitive RIA. This clone expressing the CRI produced immunoglobulin of the IgG2a subclass. Solid-phase absorption of anti-idiotype serum followed by competitive radioimmunoassay analyses revealed that all the idiotypic determinants recognized by anti-idiotype serum reside on this monoclonal antibody.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-9475
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
433-40
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Monoclonal anti-azobenzenearsonate antibodies expressing the cross-reactive idiotype: immunochemical studies show that all idiotypic determinants reside on a single molecule.
pubmed:publicationType
Journal Article