Linked Life Data

7310397

Source:http://linkedlifedata.com/resource/pubmed/id/7310397

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1982-2-12
pubmed:abstractText
Explant culture of the adult goldfish retina results in vigorous neuritic growth, provided that the optic nerve has been previously crushed in vivo. The site of new membrane addition in these growing neurites was studied with a a membrane-binding lectin Concanavalin A (Con A) in conjunction with a fluorescent indirect antibody method. Explant cultures were labeled with Con A followed by rabbit antibodies to Con A. This treatment was shown to immobilize Con A receptors while permitting growth. Twenty-four hours later, the washed preparation was treated with fluorescent goat antirabbit antibodies. The bound fluorescent antibodies appeared as a pattern of contiguous fluorescent speckles confined to the area of old growth while regions near the distal end of the neurite were not fluorescent. These results are compatible with the hypothesis that new membrane is added in the region of the growing tip of the neurite.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-3034
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
591-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Studies on the localization of newly added membrane in growing neurites.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't