Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1982-2-12
pubmed:abstractText
A substitute for the volatile solvent diethyl ether has been actively sought for the Formalin-ether sedimentation technique. Ethyl acetate has recently been shown to be a comparable substitute. In an effort to verify these findings and evaluate ethyl acetate under clinical conditions, comparison studies with 62 fresh human stool specimens were performed. Parallel concentrates with diethyl ether and ethyl acetate were prepared for each specimen, and the quantity and appearance of recovered parasite species were determined. Ethyl acetate was found comparable to diethyl ether in the quantitative recovery of parasite eggs, cysts, and larvae; no distortion or alteration of parasite morphology was observed with either solvent. More care was required, however, to completely remove the interface plugs of ethyl acetate and prevent their remixing the concentrate sediment. In addition, wet mounts prepared from ethyl acetate concentrates were occasionally obscured by liquid bubbles probably composed of remaining insoluble ethyl acetate. Clinical laboratories considering substituting ethyl acetate for diethyl ether in the Formalin-ether sedimentation technique should be aware of these problems and take the appropriate precautions to avoid them.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
483-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Clinical comparison of ethyl acetate and diethyl ether in the formalin-ether sedimentation technique.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't