Linked Life Data

7307080

Source:http://linkedlifedata.com/resource/pubmed/id/7307080

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1982-2-25
pubmed:abstractText
Acyltransferase (AT) enzyme activity was assayed biochemically and localized cytochemically in neuroblastoma monolayers to investigate the possible role of the enzyme in neuroblastoma differentiation and neurite extension. Treatment of cultures for 1 day with serum-free medium or Ro20-1724, a cyclic AMP phosphodiesterase inhibitor, induced neurite outgrowth but did not alter acyltransferase activity. Treatment for 4 days with dexamethasone or Ro20-1724 induced neurite outgrowth and a doubling of enzyme activity per cell. Chromatographic separation of lipid classes indicated that dexamethasone enhanced triacylglyceride synthesis. Acyltransferase was localized in mitochondria of neuroblastoma cells. The results show that 1) dexamethasone stimulates the storage lipid metabolic pathway in neuroblastoma cells and 2) increased acyltransferase activity is concomitant with dexamethasone-induced morphological differentiation. However, AT activity and neurite extension may not be causally related.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0045-6039
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-98
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Acyltransferase activity and cytochemical localization in differentiating neuroblastoma.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.