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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1982-2-12
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pubmed:abstractText |
The superhelical properties of chromosomal DNA from cells of a mouse sarcoma were investigated in neutral sucrose gradients containing ethidium bromide. Removal of negative supercoiling from the DNA of the sarcoma cells required a substantially higher dye concentration than was necessary in the case of DNA from cultured mouse fibroblasts. The calculated value of the mean superhelical density in malignant cells (sigma = -0.14) appears abnormally high compared with the value (sigma = -0.09) obtained for DNA of mouse fibroblasts. Chromosomal DNA from mouse sarcoma cells is therefore concluded to be highly deficient in helical turns.
|
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
|
pubmed:issn |
0304-3835
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
13
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
153-8
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:7306952-Animals,
pubmed-meshheading:7306952-Cell Nucleolus,
pubmed-meshheading:7306952-Centrifugation, Density Gradient,
pubmed-meshheading:7306952-Chromosomes,
pubmed-meshheading:7306952-DNA, Neoplasm,
pubmed-meshheading:7306952-Ethidium,
pubmed-meshheading:7306952-Mice,
pubmed-meshheading:7306952-Mice, Inbred Strains,
pubmed-meshheading:7306952-Sarcoma, Experimental
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pubmed:year |
1981
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pubmed:articleTitle |
Extremely underwound chromosomal DNA in nucleoids of mouse sarcoma cells.
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pubmed:publicationType |
Journal Article
|