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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1982-2-12
|
| pubmed:abstractText |
The synthesis of the self-complementary decadeoxynucleotide d(AATTGCAATT) is described. The phosphotriester method has been used with several modifications. Protected nucleotides have been prepared in a one-step reaction involving a new monofunctional phosphorylating agent: p-chlorophenyl-beta-cyanoethyl phosphate. Triethylammonium salts of mononucleoside 3'-phosphodiesters were obtained either by decyanoethylation of the triesters or, in the case of thymine, by a one-step reaction starting from 5'-0-methoxytritylthymidine and the mixture pyridine-para-chlorophenyl-methyl-phosphorobromidate. The usual coupling reactions were then used to prepare the decadeoxynucleotide in large quantities.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0300-9084
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
63
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
775-84
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading | |
| pubmed:year |
1981
|
| pubmed:articleTitle |
Synthesis and structural studies of a self-complementary decadeoxynucleotide d(AATTGCAATT). I.-Synthesis and chemical characterization of the decanucleotide.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|