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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1982-1-20
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pubmed:abstractText |
Poly(A)+ (polyadenylated) RNA was isolated from vitellogenic female-locus fat-body by LiCl/urea extraction and poly(U)-Sepharose 4B affinity chromatography. Agarose-gel electrophoresis of this poly(A)+ RNA under denaturing conditions shows the presence of a high-molecular-weight species (greater than 31 S, 7100 nucleotides) as the major species, which is absent from the RNA prepared from male-locust fat-body. Inclusion of this poly(A)+ RNA in a mRNA-dependent reticulocyte-lysate system directs the synthesis of polypeptides that could be immunoprecipitated with monospecific antibodies against locust egg vitellin. DNA complementary (cDNA) to the poly(A)+ RNA was synthesized, and back-hybridization of the cDNA to its template reveals a major abundant species comprising about 45% of the total poly(A)+ RNA hybridizing with R0t 1/2 of 2 x 10(-2) mol . litre-1 . s. Abundant cDNA isolated from the total cDNA hybridizes to poly(A)+ RNA with a R0t 1/2 of 9 x 10(-3) mol . litre-1 . s. There are 9.1 x 10(3) copies of vitellogenin mRNA per cell of vitellogenic female-locust fat-body, comprising 55% of the poly(A)+ RNA and equivalent to 0.7% of total cellular RNA.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-1112829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-1140218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-1176462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-1182801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-13143187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-189936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-386281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-390655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-4200179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-4213457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-424303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-455441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-4797002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-4823992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-4828327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-4850571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-4855195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-5338995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-574191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-6159243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-670199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-6986172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-711769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-728440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-73185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-738534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-7391046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-7396505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-823012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-838773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-914873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-924024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7305922-955247
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
193
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
209-16
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7305922-Animals,
pubmed-meshheading:7305922-Chromatography, Affinity,
pubmed-meshheading:7305922-DNA,
pubmed-meshheading:7305922-Electrophoresis,
pubmed-meshheading:7305922-Fat Body,
pubmed-meshheading:7305922-Female,
pubmed-meshheading:7305922-Grasshoppers,
pubmed-meshheading:7305922-Lipoproteins,
pubmed-meshheading:7305922-Nucleic Acid Hybridization,
pubmed-meshheading:7305922-Protein Biosynthesis,
pubmed-meshheading:7305922-RNA, Messenger,
pubmed-meshheading:7305922-Vitellogenins
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pubmed:year |
1981
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pubmed:articleTitle |
The preparation and characterization of locust vitellogenin messenger RNA and the synthesis of its complementary DNA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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