Linked Life Data

7298766

Source:http://linkedlifedata.com/resource/pubmed/id/7298766

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1982-1-28
pubmed:abstractText
A quantitative gas chromatographic--mass spectrometric method has been developed for the determination of glutamic acid decarboxylase (GAD) activity in subregions of rat brain. The five subregions analyzed, weighing approximately 2.51 mg each, were globus pallidus, entopeduncular nucleus, ventromedial thalamus, and substantia nigra medical and lateral. The activity of the GAD enzyme has been determined indirectly by measurement of gamma-aminobutyric acid (GABA) using gamma-[2,2-2H2] aminobutyric acid as the internal standard. Both compounds were quantitatively converted to trimethylsilyl-GABA and trimethylsilyl-[2H2]GABA in 90 min with hexamethylchlorosilane, trimethylchlorosilane, pyridine and N,O-bis(trimethylsilyl)trifluoroacetamide silylating agents. Using selective ion monitoring and electron impact ionization at 70 eV, the limit of detection was 15 ng GABA per mg tissue. This method is compared with a fluorimetric procedure.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
225
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
283-90
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Gas chromatographic-mass spectrometric determination of glutamic acid decarboxylase activity in subregions of rat brain.
pubmed:publicationType
Journal Article