Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1979-3-13
|
| pubmed:abstractText |
A 1-day rapid assay for luteinizing hormone (RLH) was developed by combined alteration of incubation temperatures and immune-complex precipitation of a commercially available LH kit. The RLH assay, compared with the standard 3-day assay, had the following characteristics: standard curve slope values of --2.47 and --2.314; assay sensitivity of 5.5 and 3.8 mIU/ml of serum; average coefficients of variation for the standard curves of 13.6% and 14.1% and for serum controls of 10.6% and 13.1%, respectively. Two consecutive RLH assays of split samples were highly correlated (r = 0.9908; P less than 0.001), as were split aliquots measured by RLH or the 3-day assay (r = 0.993; P less than 0.001). When 52 samples from portions of 5 normal menstrual cycles were assayed in both systems, agreement was again high (r = 0.9871; P less than 0.001). Representative curves of both RLH and standard LH assays, together with other data, are presented to show the feasibility of applying this method for timing of artificial insemination.
|
| pubmed:keyword |
http://linkedlifedata.com/resource/pubmed/keyword/Artificial Insemination,
http://linkedlifedata.com/resource/pubmed/keyword/Biology,
http://linkedlifedata.com/resource/pubmed/keyword/Endocrine System,
http://linkedlifedata.com/resource/pubmed/keyword/Examinations And Diagnoses,
http://linkedlifedata.com/resource/pubmed/keyword/Gonadotropins,
http://linkedlifedata.com/resource/pubmed/keyword/Gonadotropins, Pituitary,
http://linkedlifedata.com/resource/pubmed/keyword/Hormones,
http://linkedlifedata.com/resource/pubmed/keyword/Infertility,
http://linkedlifedata.com/resource/pubmed/keyword/Laboratory Examinations And Diagnoses,
http://linkedlifedata.com/resource/pubmed/keyword/Laboratory Procedures,
http://linkedlifedata.com/resource/pubmed/keyword/Luteinizing Hormone--analysis,
http://linkedlifedata.com/resource/pubmed/keyword/Ovulation Detection,
http://linkedlifedata.com/resource/pubmed/keyword/Physiology,
http://linkedlifedata.com/resource/pubmed/keyword/Prospective Studies,
http://linkedlifedata.com/resource/pubmed/keyword/Reproduction,
http://linkedlifedata.com/resource/pubmed/keyword/Reproductive Technologies,
http://linkedlifedata.com/resource/pubmed/keyword/Research Methodology,
http://linkedlifedata.com/resource/pubmed/keyword/Studies
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0015-0282
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
30
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
648-53
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:otherAbstract |
PIP: The characteristics of a rapid 1-day assay for luteinizing hormone (RLH), developed by combined alteration of incubation temperatures and immune-complex precipitation of commercially available LH kit, are described, and its application to a group of patients scheduled for artificial insemination (AI) as an ovulation predictor is presented. The modification of the commercial LH kit is covered in a stepwise manner textually. Compared with the standard 3-day LH kit, the RLH assay had the following characteristics: standard curve slope values of -2.47 and -2.314; assay sensitivity of 5.5 and 3.8 3.8 mIU of serum; average coefficients of variation for the standard curves of 13.6% and 14.1% and for serum controls of 10.6% and 13.1%, respectively. Split correlations of the RLH assay and correlations of the RLH and standard 3-day assay were both highly correlated (P .001). When 52 samples from 5 normal menstrual cycles were assayed in both the 3-day and the RLH systems, correlation was again P .001. When used as an ovulation predictor in 6 subjects for AI, 4 patients conceived, 2 during the first cycle in which they were inseminated and 2 in the second consecutive insemination cycle.
|
| pubmed:meshHeading |
pubmed-meshheading:729826-Adult,
pubmed-meshheading:729826-Female,
pubmed-meshheading:729826-Humans,
pubmed-meshheading:729826-Infertility, Female,
pubmed-meshheading:729826-Insemination, Artificial,
pubmed-meshheading:729826-Luteinizing Hormone,
pubmed-meshheading:729826-Ovulation Detection,
pubmed-meshheading:729826-Radioimmunoassay,
pubmed-meshheading:729826-Time Factors
|
| pubmed:year |
1978
|
| pubmed:articleTitle |
The use of a one-day luteinizing hormone assay for timing of artificial insemination in infertility patients.
|
| pubmed:publicationType |
Journal Article
|