Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1981-12-15
pubmed:abstractText
In ACD solution which contains PEP and sucrose was used as a preservative solution as well as a medium for rejuvenating depleted erythrocytes. 2,3-DPG and ATP in whole blood or red cell concentrates were increased effectively by incubating the cells with the solution at 37 degrees C for 30-60 min. The transport of PEP through the erythrocyte membrane was essential to the increase of 2,3-DPG and ATP. During storage of the cells at 4 degrees C in the presence of PEP, no increase of 2,3-DPG and ATP was observed because no transport of PEP into the cells occurred. By incubation at 37 degrees C for 30 min at the end of various storage periods, however, the levels of ATP and 2,3-DPG in the cells were raised. Addition of ascorbic acid, purine or purine nucleosides to the ACD-sucrose-PEP solution improved the PEP effect of maintaining ATP and/or 2,3-DPG in erythrocytes during storage at 4 degrees C.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0361-7742
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
577-94
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Regeneration of 2,3-bisphosphoglycerate and ATP of stored erythrocytes by phosphoenolpyruvate; a new preservative for blood storage.
pubmed:publicationType
Journal Article