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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1981-12-15
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pubmed:abstractText |
In ACD solution which contains PEP and sucrose was used as a preservative solution as well as a medium for rejuvenating depleted erythrocytes. 2,3-DPG and ATP in whole blood or red cell concentrates were increased effectively by incubating the cells with the solution at 37 degrees C for 30-60 min. The transport of PEP through the erythrocyte membrane was essential to the increase of 2,3-DPG and ATP. During storage of the cells at 4 degrees C in the presence of PEP, no increase of 2,3-DPG and ATP was observed because no transport of PEP into the cells occurred. By incubation at 37 degrees C for 30 min at the end of various storage periods, however, the levels of ATP and 2,3-DPG in the cells were raised. Addition of ascorbic acid, purine or purine nucleosides to the ACD-sucrose-PEP solution improved the PEP effect of maintaining ATP and/or 2,3-DPG in erythrocytes during storage at 4 degrees C.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0361-7742
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
577-94
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:7291198-2,3-Diphosphoglycerate,
pubmed-meshheading:7291198-Adenosine Triphosphate,
pubmed-meshheading:7291198-Blood Specimen Collection,
pubmed-meshheading:7291198-Diphosphoglyceric Acids,
pubmed-meshheading:7291198-Erythrocytes,
pubmed-meshheading:7291198-Glycolysis,
pubmed-meshheading:7291198-Humans,
pubmed-meshheading:7291198-Kinetics,
pubmed-meshheading:7291198-Phosphoenolpyruvate
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pubmed:year |
1981
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pubmed:articleTitle |
Regeneration of 2,3-bisphosphoglycerate and ATP of stored erythrocytes by phosphoenolpyruvate; a new preservative for blood storage.
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pubmed:publicationType |
Journal Article
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