Linked Life Data

7288296

Source:http://linkedlifedata.com/resource/pubmed/id/7288296

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1981-12-22
pubmed:abstractText
A microanalytical procedure for the determination of trace amounts of lysophosphatidylethanolamine and other amine-containing lipids is described. The technique involves reaction of lipid extracts with fluorescamine to give fluorescent derivatives of lipids containing a free amino group, separation of the products by thin-layer chromatography, and quantification by fluorescence spectroscopy after elution from the adsorbent. Amounts of 100 pmol are easily determined, even in the presence of large amounts of other phospholipids, and 30-40 samples can be analyzed per day. Use of the procedure is illustrated with rat liver mitochondria undergoing a phospholipase A2-dependent increase in inner membrane permeability due to treatment with Ca2+ plus N-ethylmaleimide.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
882-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Quantification of lysophosphatidylethanolamine in the nanomole range.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't